Establishment of ALI-Macrophage co-culture system with porcine alveolar and mouse bone marrow derived macrophages
In ALI cultures, basal cells of the upper respiratory epithelial cells, mainly tracheal and bronchial basal epithelial cells, are used. By using various growth supplements, these cells are allowed to differentiate. This method closely mimics the in vivo situation. However, the lack of some of the innate immune cells in this system, which are commonly present in the upper respiratory tract, is a drawback in fully representing this the in vivo situation. In the PIGSs project we succeeded in overcoming hurdle by the establishment of an ALI-macrophage co-culture system.