Establishment of ALI-Macrophage co-culture system with porcine alveolar and mouse bone marrow derived macrophages

In ALI cultures, basal cells of the upper respiratory epithelial cells, mainly tracheal and bronchial basal epithelial cells, are used. By using various growth supplements, these cells are allowed to differentiate. This method closely mimics the in vivo situation. However, the lack of some of the innate immune cells in this system, which are commonly present in the upper respiratory tract, is a drawback in fully representing this the in vivo situation. In the PIGSs project we succeeded in overcoming hurdle by the establishment of an ALI-macrophage co-culture system. 


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Webinar organized by Schothorst Feed Research: The role of gut microbiota on welfare of pigs.

The microbiota-gut-brain axis (MGBA) plays a big role in pig welfare: feeding and social behaviour, stress, fear and memory. A better understanding of the influence of the MGBA on welfare is needed to develop innovative feeding strategies that can improve both animal performance, health and welfare. A webinar organized by Schothorst Feed Research will present latest data on the role of gut microbiota on welfare of pigs. 


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How is COVID-19 affecting the pork and commodity markets?

The health crisis caused by COVID-19 is affecting the pig sector, both in the French and international markets. Companies in the sector are experiencing major changes in their operations. Pig prices are falling due to lower demand from slaughterhouses, affected by labor shortages and problems accessing foreign markets. Despite the strong demand for exports to third countries, these new elements are limiting the price of pork for the time being.


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Identification of novel virulence factors of Streptococcus suis:

Use of precision-cut lung slices (PCLS) and air-liquid interface (ALI) cultures as in vitro models to reduce experimental animal infection


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Wageningen Bioveterinary Research supports large-scale testing corona patients in the Netherlands

Samples to test corona patients for the COVID-19 virus are sent to the Wageningen Bioveterinary Research (WBVR) laboratory as of Monday 30 March. This constitutes a significant increase in the testing capacity in the Netherlands.

 

 


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Covid-19 can put the human teams on our farms at risk.

Covid-19 can put the human teams on our farms at risk. A very interesting review was published by Van Doremalen et al on coronavirus persistence on different materials and in aerosols can help us implement measures on swine farms to reduce the human risk of infection.


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Data presented from the PIGSs project at the Jornadas de porcino in Barcelona

Dr Virginia Aragon from IRTA-CReSA shared with porcine veterinarians an update of the studies on tonsil microbiota and the role in Streptococcus suis disease. 


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PIGSs researchers at the Cambridge Science Festival

PIGSs researchers at the Cambridge Science Festival reveal latest insights in Streptococcus suis.


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Dedicated PIGSs meeting on: Identification of new or uncharacterised mutants.

To allign ongoing research activities in the PIGSs project on virulence (work package 2), and identify new or uncharacterised mutants, an ad hoc meeting was organized today (11 February 2020) by Prof. Peter Valentin-Weigand at Wageningen University and Research.


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Course Gut Health in Pigs (The influence of nutrition and immunology) organized by the Wageningen Academy

Maintaining or promoting intestinal health is of crucial importance for optimal production efficiency, overall health and promoting the welfare of production animals such as pigs and poultry. Are you wondering what factors affect gut health? This course was organized by Wageningen Academy on 3 February 2020. 


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Primary porcine respiratory epithelial cell models used in the PIGSs project to study host–pathobiont interactions in the respiratory tract: ALI culture.

There is a growing need for physiologically relevant models of the respiratory epithelium, but recreating these complex functions in vitro is a considerable challenge. In the PIGSs project, Air-Liquid-Interface (ALI) cultures from primary porcine tracheal and bronchial epithelial cells are developed as in vitro culture tools that closely mimics the in vivo situation in pigs. 


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Primary porcine respiratory epithelial cell models used in the PIGSs project to study host–pathobiont interactions in the respiratory tract: PCLS model.

The PIGSs project aims to improve our current understanding of the interplay of commensals, pathobionts, and keystone pathogens in the respiratory tract. Studies to dissect these complex processes should be carried out in respective animal models, e.g., in pigs, or under conditions which most closely mimic in vivo conditions. 


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Characterization of virulence genes in Streptococcus suis using CRISPRi and organoid models

Streptococcus suis is a bacterial pathogen which is a major cause of mortality and economic losses in the pig farming industry. Moreover, it is also a zoonotic pathogen causing human infections worldwide. Almost 100% of pig farms worldwide have carrier animals, but the factors leading to invasive disease are not well understood. Genetic variation within S. suis complicate the development of effective control strategies so that to date, no efficacious, cross-protective vaccines exist for S. suis. The overall aim of this research project is to unravel the host-pathogen interactions that take place during the infection process and thereby aid the development of effective vaccines, anti-virulence strategies and diagnostic tools to combat S. suis infections.


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Mutants constructed in selected candidate virulence genes in S. suis strains

In the PIGSs project we will determine which S. suis are conditionally essential for survival and growth in the ecological niches within the host mainly using data from (1). Transcriptomics under in vivo mimicking conditions, (2). TnSeq mutagenesis under in vivo mimicking conditions and (3). Genomics data from the collection of clinical isolates sequenced during the initial phases of the project.


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Global sample collection of S. suis strains.

Global sample collection of S. suis strains undertaken in PIGSs completed. 


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Presentation on the power of microbial diversity in humans and animals at the Pint of Science festival

Dr. Flor Correa gives a talk on “The power of (microbial) diversity“ to the lay public at the Pint of Science festival, Barcelona. The microbiota is the group of microorganisms that co-exist in different body sites without harming the host, capable of contributing with essential functions for life.  But, what happen when the bacterial communities are altered? How does the microbiota composition affect to the susceptibility to diseases? These were some of the questions Ishe explained in an unusual environment: the pub.


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Porcine ileal organoids grown by Wageningen University for advanced in vitro studies in the PIGSs project.

Examples of ileal organoids from pigs grown in 3D matrigel from partner Wageningen University. This advanced in vitro model will be used to analyse the mechanism of host-cell interactions and virulence in work package 2 of the PIGSs project


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Results on in vivo transcriptomes of Streptococcus suis published in Virulence.

Principal Investigators from the Wageningen University groups of Animal Sciences and Host-Microbe Interactions jointly published their collaborative work on in vivo transcriptomes of Streptococcus suis in Virulence. 


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Members of PIGSs consortium meet in Sitges

The PIGSs consortium meeting was a great succes! Virginia Aragon and Jordy Casal are the workpackage leaders from IRTA-CReSA and shared there experience on their website.


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PIGSs represented on meeting of the National Institute for Agrifood Research (INIA)

On April 11, 2019, Virginia Aragon presented at the National Institute for Agrifood Research (INIA).

 


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