Porcine ileal organoids grown by Wageningen University for advanced in vitro studies in the PIGSs project.

Examples of ileal organoids from pigs grown in 3D matrigel from partner Wageningen University. This advanced in vitro model will be used to analyse the mechanism of host-cell interactions and virulence in work package 2 of the PIGSs project ("Virulence").

Figure A) mature organoid with crypt formation.

Figure B) Hematoxylin and eosin (H&E) stained organoids showing Paneth cells containing granules. Paneth cells are key epithelial cells that provide an antimicrobial barrier and maintain integrity of the small-intestinal stem cell niche.

Figure C) Histological Periodic acid-Schiff (PAS) staining revealing goblet cells and secreted mucus in lumen of organoid. Goblet cells are simple columnar epithelial cells that secrete gel-forming mucins. 

Figure D) Chromogranin A staining for enteroendocrine cells in a 2D monolayer of organoid cells (van der Heeet al., 2018).